A total of 100 samples of meat cuts and minced meat (50 of each) were collected from different localities of different sanitation levels from Gharbia governorate. For moulds, the prevalence of isolated species were Aspergillus (52.9% and 45.6%), Penicillium (21.1% and 27.2%), Acremonium (3.5 % and 6.8%), Alternaria (11.7% and 12.6%), and Curvularia (4.7% and 0.9%) for meat cuts and minced meat respectively. Endomyces and Eupenicillium were isolated by a percent of (2.3% and 3.5%) respectively for meat cuts, while Chaetomium and fusarium were (3.9% and 2.9%) for minced meat respectively. The result showed that the Aspergillus and Penicillium were the most isolated mould species, furthermore Aspergillus was further identified to A.flavus, A.niger, A.terreus, A. aculeatus and A.fumigatus. A. niger was the most predominant specie (48.8% and 59.5%) for meat cuts and minced meat respectively. Penicillium also was further identified to P. fellutanum, P .citreonigrum, P. aurantiogriseum and P. thomii. P. aurantiogriseum was the most predominant species (33.3% and 32.1%) for meat cuts and minced meat respectively. For yeasts, the prevalence of isolated species were Candida (50% and 48.4%), Rhodotorula (13.3% and 18.7%), Saccharomyces (16.6% and 12.5%) and Torulopsis (20% and 15.6%) for meat cuts and minced meat respectively. The results also indicated the mean concentration of aflatoxin B1 was (7.8±2.2 and 3.4±0.5) and Ochratoxin A (3.9±0.8 and 8.1±1.9) for meat cuts and minced meat respectively.