Study on fungal contamination of somechicken meat products withspecial reference to 2 the use of PCR for its identification
Conference,Veterinary Medical Journal – Giza vol. December 2014/12/17 vol.60 • 2014
Publication Information
Authors
Shaltout, Fahim; El-diasty, E,M. ;Elmesalamy, M. and Elshaer, M
Keywords
A. parasiticus, chicken meat, PCR, sequences,P.purpurogenum.
Journal
Conference,Veterinary Medical Journal – Giza vol. December 2014/12/17 vol.60
Publisher
faculty of veterinary medicine cairo university
Volume
60
Issue
December 2014/12/17
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Fahim Aziz El-Dein Mohamed Shaltout_nskht almjlt bd altshyh alakhyr (1).pdf
Abstract
This work was carried out to evaluatethe fungalcontamination of chicken meat products 13 sold in local markets as well as identification of some isolated moulds using PCR 14 technique.Foridentification of the isolated moulds,samples were subjected to 15 mycologicalexaminationusing themorphological (macroscopic and 16 microscopic)characterization.Molecular identification using (ITS) was carried out for 17 isolatedAspergillus and Penicillium species. The average total mould counts in the 18 examined samples ofchickenluncheon, chicken pane and chicken minced meat were 3.1 x 19 102 ± 0.82 x 102, 7.4 x 102 ± 5.4 x 102 and 1.7 x 102 ± 0.16 x 102cfu/gm, respectively. In the 20 examined samples, 9mould genera were identified. The identified mould genera 21 wereAspergillus, Eurotium,Penicillium,Geotrichum,Fusarium,Cladosporium, Mucor, 22 Eupencillium and Acremonium species. The isolated species ofAspergillus parasiticus and 23 Penicillium purpurogenumwere subjected to PCR identification, and were sequenced in 24 bothdirections. Sequences were analysed and aligned by Clustal method using the program 25 of DNAstar (Laser-gene, Wisconsin, USA). 26
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