105 random samples of frozen sausages, collected from different localities (Benha city, Centers of kaliobia governorate and villages)
with 35 for each locality to evaluate the bacterial quality and the hygienic health hazard of them. The mean value of APC, Psychrotrophic,
Enterobacteriaceae, Coliform and Staphylococcus counts in frozen sausage samples collected from Benha city were 3.62 × 104 ±
0. 19 ×104; 2.10 × 104 ± 0. 13 ×104; 1.53×102 ± 0. 09 ×102; 0.92 × 102 ± 0. 13 ×102 and 1.62 × 102 ± 0.10 × 102, respectively; for frozen
sausage samples collected from different centers of kaliobia governorate were 5.88 × 104 ± 0.17 × 104; 2.98 × 104 ± 0.11×104; 2.36 ×
102 ± 0.11 × 102; 1. 93 × 102 ± 0.10 × 102 and 2.10 × 102 ± 0.09 × 102, respectively; for frozen sausage samples collected from different
villages were 7.91 × 104 ± 0. 16 × 104; 5.32 × 104 ± 0. 22 × 104; 4.84 × 102 ± 0.24 × 102; 4. 33 × 102 ± 0.22 × 102 and 3.23 × 102 ± 0.16
× 102, respectively. SET- RPLA test revealed that, seven strains out of 10 random examined strains (70.0%) were enterotoxigenic and
classified according to type of toxin into (4A; 1B; 2A & C).