Out of 100 samples taken from the surface of the skin of poultry carcasses in poultry slaughterhouses, 54 samples were positive to Salmonella enterica serovar enteritidis with a percentage of (54%). 100 fecal samples were taken from human stools of workers in contact with poultry in poultry slaughterhouses at Kalyobia Governorat, suffering from diarrhea and/or fever. Salmonella enterica serovar enteritidis was represented in 42 samples with a percentage of (42%). Phage typing of isolated strains from poultry and poultry attendant demonstrated five strains 1,4,6, 21 and 28 having the possibility of cross infection between poultry men and poultry carcasses. Antimicrobial sensitivity test proved that those Salmonella enterica isolated strains were sensitive to Ampicillin (10 µg), Amoxicillin (20 µg), Gentamycin (10 µg), Kanamycin (30 µg), Nitrofurantoin (300 µg), and Cephalothin (30 µg) and they were medium resistant to Streptomycin (10 µg), and Tetracycline (30 µg). The public health significance of the isolated strains was discussed.