Staff Members - Benha University
Plasmid profile analysis of Salmonellae isolated from some meat products.
Benha veterinary medical journal • 2011
Publication Information
Authors
Edris, A.M.; Shaltout, F.A. ;Salem, G.H. and El-Toukhy,E.I.
Keywords
Luncheon, Plasmid, Salmonellae, Sausage, Minced Meat.
Journal
Benha veterinary medical journal
Publisher
faculty of veterinary medicine , benha university
Volume
Not Available
Issue
Not Available
Pages
172-178,
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Fahim Aziz Eldin Mohamed Shaltout_Fahim Aziz Eldin Mohamed Shaltout_949c74de8b116b3992b1a951d635d1be8502 (1).pdf
Abstract
A total of 300 random samples of luncheon, sausage and frozen minced meat (100 of each) were
collected from different supermarkets at Qalyubia governorate. The samples were transferred directly
to the laboratory under strict hygienic conditions and bacteriologically examined for detection and
plasmid profile analysis of salmonellae. This work determined the plasmid profiles of the salmonella
serovars by using ethidium bromid stained 1% agarose gel electrophoresis. In this study, plasmid
analysis revealed that the isolated salmonella serotypes carry different numbers of plasmids with
variable molecular weights ranged from 1233.18 to 15280.82 base pair (bp). The obtained results
revealed that S. typhi, S. typhimurium and S. enteritidis contain a common molecular weight band at
7532.35 bp. and the number of plasmids ranged from 4 to 5 bands. Moreover, S. typhi and S.
typhimurium sharing the same band at 1233.18 bp. In the current study, S. enteritidis and S.
typhimurium were recognized to have common band at 1631.12 bp. The obtained results revealed that
S. typhi, and S. enteritidis have 4 bands but S. typhimurium have 5 bands.
collected from different supermarkets at Qalyubia governorate. The samples were transferred directly
to the laboratory under strict hygienic conditions and bacteriologically examined for detection and
plasmid profile analysis of salmonellae. This work determined the plasmid profiles of the salmonella
serovars by using ethidium bromid stained 1% agarose gel electrophoresis. In this study, plasmid
analysis revealed that the isolated salmonella serotypes carry different numbers of plasmids with
variable molecular weights ranged from 1233.18 to 15280.82 base pair (bp). The obtained results
revealed that S. typhi, S. typhimurium and S. enteritidis contain a common molecular weight band at
7532.35 bp. and the number of plasmids ranged from 4 to 5 bands. Moreover, S. typhi and S.
typhimurium sharing the same band at 1233.18 bp. In the current study, S. enteritidis and S.
typhimurium were recognized to have common band at 1631.12 bp. The obtained results revealed that
S. typhi, and S. enteritidis have 4 bands but S. typhimurium have 5 bands.