Staff Members - Benha University
Aerobic Plate Count of Contaminants and Molecular Characterization of Eschereichia Coli in Raw Chicken Meat in Ismailia, Egypt
JOURNAL OF VETERINARY HEALTHCARE • 2020
Publication Information
Authors
El -Sayed¹
, A Afify¹
, Fahim A Shaltout¹,*, Zakaria, I. M¹
Keywords
Chicken meat, Identification, characterization, E.coli , PCR
Journal
JOURNAL OF VETERINARY HEALTHCARE
Publisher
CC-license
Volume
2
Issue
2
Pages
23-30
publication.type
International
Paper Link
Open Link
Supplementary Materials
Fahim Aziz Eldin Mohamed Shaltout_JVHC-20-3477.pdf
Abstract
A total number of 100 samples from ten random broiler chicken carcasses (breast and thigh) were
collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The mean values of
Enterobacteriacae count were 5.9x104±9.7x103 cfu/g and 7.1x 104 ± 1.1x104 cfu/g for chicken breast and
thigh samples respectively. The prevalence of E.coli were 12% and 9% breast and thigh samples examined,
respectively. They are serologically identified as 33.35 and 22.2% O157:H7 (EHEC) , 16.6% and 11.1% O114:H21
(EPEC), 16.6% and 33.3 %O127:H6 (ETEC) , 0% and 0% O126 (ETEC) and 33.3% and 0% O26 (EHEC) for
breast and thigh samples, respectively. The incidence of E.coli O157:H7 was 100% in both serological and PCR
methods from biochemical positive E.coli samples. Culture is specific and cheap whereas PCR is sensitive and
expensive, hence, we recommend both culture and molecular methods, which improve sensitivity and
specificity, to enhance detection of foodborne pathogens including E.coli .
collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The mean values of
Enterobacteriacae count were 5.9x104±9.7x103 cfu/g and 7.1x 104 ± 1.1x104 cfu/g for chicken breast and
thigh samples respectively. The prevalence of E.coli were 12% and 9% breast and thigh samples examined,
respectively. They are serologically identified as 33.35 and 22.2% O157:H7 (EHEC) , 16.6% and 11.1% O114:H21
(EPEC), 16.6% and 33.3 %O127:H6 (ETEC) , 0% and 0% O126 (ETEC) and 33.3% and 0% O26 (EHEC) for
breast and thigh samples, respectively. The incidence of E.coli O157:H7 was 100% in both serological and PCR
methods from biochemical positive E.coli samples. Culture is specific and cheap whereas PCR is sensitive and
expensive, hence, we recommend both culture and molecular methods, which improve sensitivity and
specificity, to enhance detection of foodborne pathogens including E.coli .