100 random samples of local and imported canned Tuna and Mackerel fish (25 of each) were analyzed for detection and determination of biogenic amines residues (histamine, tyramine and putrescine) by High Performance Liquid Chromatography(HPLC). The obtained results revealed that the average concentration level of histamine, tyramine, and putrescine (mg/kg) were 22.86±0.72, 0.274±0.035 & 1.884±0.181, 19.48±0.64, 0.184±0.177& 1.72±0.076, 36.72±1.807, 0.393±0.048 & 2.1±0.104 , 29.04±1.46, 0.368±0.047 and 1.894±0.115 for local and imported tuna and mackerel samples ,respectively. All Samples of both canned tuna and mackerel were acceptable for histamine, tyramine and putrescine Except 8% of canned mackerel exceeded permissible limits for histamine according to Egyptian Organization of Standardization (EOS,2005) .