Five samples of beef escalope panee were randomly collected from different localities in Qalyubia governorate in order to clarify the effect of preservative nisin on the survival of Listeria monocytogenes (L. monocytogenes) and suggest the concentrations of Nisin that could be useful in controlling L. monocytogenes. The inhibitory effect of Nisin at concentration of 10, 30 and 50 ppm on the survival of L. monocytogenes (5×106/g) inoculated into beef escalope pane. It was noticed that the treatment of inoculated beef escalope panee with nisin at concentration of 30 or 50 ppm for 24 hours was useful in controlling L. monocytogenes.