Incidence of Staphylococcus enterotoxins in some meals served in Egyptian hotel
Benha veterinary medical Journal • 2021
Publication Information
Authors
Osama. M. Abbass , Saad. M. Saad, Mohammed A. Hassan, Walid. S. Arab
Keywords
S.aureus , entertoxin, PCR
Journal
Benha veterinary medical Journal
Publisher
BVMJ
Volume
40
Issue
1
Pages
124-127
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Walid Sobhy Saber Elsaid Arab_Staphylococcus in hotels.pdf
Abstract
120 random samples of beef kofta, chicken panne, and fish based meal (sushi) (40 of each) , served in Egyptian hotels located in Cairo governorate, Egypt were collected to determine of S.Aureus counts, isolation , identifications and detection of their enterotoxins
in the examined samples.
The obtained results revealed that S. epidermidis, S. intermedius, S. saprophyticus and S. xylosus were recorded in (25 %), (2.5%), (0%) and (12.5 %) of Kofta , while for chicken panne were detected (37.5 %), (10 %), (7.5%) and (2.5 %) , in add to (45 %), (17.5%), (5%) and (10 %) were detected in Sushi fish samples, furthermore S. aureus was detected in 13(32.5%), 21(52.5%) and 27(67.5%) of the examined kofta, panne and sushi samples
Regarding to the edibility of the examined ready to eat ( RTE) samples in relation to its content of S. aureus, 11(27.5%), 21(52.5%) and 27(67.5%) of the examined kofta, panne and sushi samples respectively, were rejected for exceeding S. aureus permissible limit (not more than 102 ).
The results recorded als the main values of S . aureus counts in the examined kofta, panne and sushi samples were 9.41×102± 2.12×102, 3.27×103± 0.54×103 and 5.86×103± 0.97×103 cfu/g, respectively.
Moreover the incidence for S. aureus enterotoxins positive samples, revealed that SE-A was 1(2.5%), 2(5%) and 4(10%) of the examined kofta, panée and sushi samples, respectively; while SE-B was detected in 1(2.5%) of chicken panée only , while not detected in kofta and sushi samples, SE-D was detected in 1(2.5%) each of kofta and sushi sample , while not detected in panne, SE-A+C was detected in 1(2.5%) of kofta samples while not detected in panne and sushi samples, SE-A+D were detected in 1 (2.5%) each of panne and sushi samples while not detected in kofta samples. Overall, 3(7.5%), 4(10%) and 6(15%) of the examined kofta, panne and sushi .
Wherever to the highly obtained results of the S. aureus prevalence, especially enterotoxigenic strains; it encourages investigation of the antibiotic resistance profile molecularly against erythromycin (ermA), gentamicin (aac 6-aph 2), methicillin (mecA) and vancomycin (vanA) resistance genes in randomly collected five S. aureus isolates of each sample and results revealed that out of the examined 15 isolates, 8(53.3%), 5(33.3%), 4(26.6%) and 2(13.3%) were harbored ermA, aac 6-aph 2, mecA and vanA resistance genes, where sushi isolates had the highest prevalence of antibiotic resistance followed by panée and kofta samples, respectively
in the examined samples.
The obtained results revealed that S. epidermidis, S. intermedius, S. saprophyticus and S. xylosus were recorded in (25 %), (2.5%), (0%) and (12.5 %) of Kofta , while for chicken panne were detected (37.5 %), (10 %), (7.5%) and (2.5 %) , in add to (45 %), (17.5%), (5%) and (10 %) were detected in Sushi fish samples, furthermore S. aureus was detected in 13(32.5%), 21(52.5%) and 27(67.5%) of the examined kofta, panne and sushi samples
Regarding to the edibility of the examined ready to eat ( RTE) samples in relation to its content of S. aureus, 11(27.5%), 21(52.5%) and 27(67.5%) of the examined kofta, panne and sushi samples respectively, were rejected for exceeding S. aureus permissible limit (not more than 102 ).
The results recorded als the main values of S . aureus counts in the examined kofta, panne and sushi samples were 9.41×102± 2.12×102, 3.27×103± 0.54×103 and 5.86×103± 0.97×103 cfu/g, respectively.
Moreover the incidence for S. aureus enterotoxins positive samples, revealed that SE-A was 1(2.5%), 2(5%) and 4(10%) of the examined kofta, panée and sushi samples, respectively; while SE-B was detected in 1(2.5%) of chicken panée only , while not detected in kofta and sushi samples, SE-D was detected in 1(2.5%) each of kofta and sushi sample , while not detected in panne, SE-A+C was detected in 1(2.5%) of kofta samples while not detected in panne and sushi samples, SE-A+D were detected in 1 (2.5%) each of panne and sushi samples while not detected in kofta samples. Overall, 3(7.5%), 4(10%) and 6(15%) of the examined kofta, panne and sushi .
Wherever to the highly obtained results of the S. aureus prevalence, especially enterotoxigenic strains; it encourages investigation of the antibiotic resistance profile molecularly against erythromycin (ermA), gentamicin (aac 6-aph 2), methicillin (mecA) and vancomycin (vanA) resistance genes in randomly collected five S. aureus isolates of each sample and results revealed that out of the examined 15 isolates, 8(53.3%), 5(33.3%), 4(26.6%) and 2(13.3%) were harbored ermA, aac 6-aph 2, mecA and vanA resistance genes, where sushi isolates had the highest prevalence of antibiotic resistance followed by panée and kofta samples, respectively
Staff Members - Benha University