Background: The success of heath organizations depends on not only capacity to survive but even more so on its ability to continuously adapt and change. Agile nurse leaders require not to be afraid of change, but to renew their selves regularly to provide competitive advantage. Aim: The study aimed to determine the effect of educational program about head nurses' agile leadership on staff nurses' workplace spirituality and job reputation. Design: A quasi-experimental research design Setting: The study was conducted in all units at Benha University Hospital. Subjects: Two groups were included in this study; convenient sample (head nurses group; n=80) and purposive sample (staff nurses group; n=310). Tools: Four tools were used in this study; (1): Agile Leadership Knowledge Test, (2): Agile Leadership Practice Self-administered Questionnaire, (3): Workplace Spirituality Questionnaire and (4): Job Reputation Questionnaire. Results: The study findings revealed that there were statistical significant improvement in head nurses' agile leadership knowledge and practice, and staff nurses' perception level regarding workplace spirituality and job reputation after implementation of educational program. Conclusion: There were statistically significant positive correlation among head nurses' agile leadership knowledge and practice and staff nurses' workplace spirituality and job reputation scores. Recommendation: Supporting organizational culture that contribute to the growth of the concept and strategies of agile leadership and its effect on the workplace spirituality and job reputation.