The present study aimed to test the impact of an online E-training program for developing professional Attitudes, self-efficacy beliefs and literacy towards Science, Technology, Engineering and Mathematics (STEM) on female middle school’s science teachers. The study used the experimental design of one group pretest posttest. Studying group included (16) Female middle school’s science teachers in Wadi Al-Dawasir Governorate, Riyadh. The study applied three scales: STEM Professional Attitudes scale, STEM Self-efficacy beliefs scale and STEM Literacy for science teacher.The results indicates that there are statistically significant differences at the level (α = 0.05) in STEM Professional Attitudes scale between the pretest posttest in favor of posttest , there are statistically significant differences at the level (α = 0.05)in STEM Self-efficacy beliefs scale between the pretest posttest in favor of posttest and there are statistically significant differences at the level (α = 0.05)in STEM Literacy scale test in favor of posttest.The results were discussed and the study presented recommendations and suggestions depending on the results.