This study examined the effect of dual-frequency ultrasound (DFU) pretreatment on thermodynamics and kinetics of sunflower-meal protein (SMP) using alcalase to improve efficiency in enzymolysis process. The concentration of hydrolyzed protein and kinetic parameters after traditional pretreatment (control) were investigated and compared with DFU-assisted enzymolysis. The results indicated that DFU-pretreatment enhanced SMP-enzymolysis efficiency at different substrate and enzyme concentrations, temperature, and pH. Kinetics analysis showed DFUpretreatment reduced the Michaelis constant by 11.29%, while the apparent breakdown rate constant increased by 1.96%, indicating DFU-pretreatment improved the affinity among substrate and enzyme. The rate constants for DFU-pretreatment were increased by 45.96, 26.92, 21.14, and 27.89% at 293, 303, 313, and 323 K, respectively (p < .05). On Arrhenius kinetics, DFU reduced the activation energy, enthalpy and entropy by 24.28, 26.13, and 9.10%, respectively (p < .05). DFU had slight influence on Gibbs-free energy when temperature increased from 293 to 323 K.