Staff Members - Benha University
Antibiotic Resistance, Molecular typing, Biofilm Formation in Enterococcai Isolates Causing Urinary Tract Infection
JOURNAL OF PURE AND APPLIED MICROBIOLOGY • 2016
Publication Information
Authors
Eman A. El-Masry1, Elham T. Awad 1 and Mohamed H. Yassin2*
Keywords
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Journal
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
Publisher
Not Available
Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
Vancomycin resistant Enterococci (VRE) are becoming a major emergence problem
concern in urinary tract infection (UTI) at Shpin Elkom Teaching Hospitals, Menofia
University. The aim of study to estimate the extent of spread of Enterococcai infection as
well as vancomycin-resistant. Enterococci were isolated from 200 UTI patients and
identified as E. faecalis by biochemical tests using the API test kit. VRE was determined
by agar disc diffusion. The results showed that, 34 (26.5%) Enterococci were isolated
from UTI patients. E. faecalis (64.7%) was the most common isolate followed by E.
faecium (35.3%) out of 34 UTI patients. Among the UTI 64.7% Enterococci were
phenotypically resistant to Vancomycin. Vancomycin resistance genes (vanA, vanB) and
biofilm formation (esp gene) were detected by amplifying the respective genes by PCR.
This study shows increase prevalence of Enterococci and VRE isolates as a cause of
urinary tract infection in our hospitals. It should be still careful of appropriate use of
antibiotics such as vancomycin. Detection and containment of VRE, a more targeted,
systematic approach is needed among patients.
concern in urinary tract infection (UTI) at Shpin Elkom Teaching Hospitals, Menofia
University. The aim of study to estimate the extent of spread of Enterococcai infection as
well as vancomycin-resistant. Enterococci were isolated from 200 UTI patients and
identified as E. faecalis by biochemical tests using the API test kit. VRE was determined
by agar disc diffusion. The results showed that, 34 (26.5%) Enterococci were isolated
from UTI patients. E. faecalis (64.7%) was the most common isolate followed by E.
faecium (35.3%) out of 34 UTI patients. Among the UTI 64.7% Enterococci were
phenotypically resistant to Vancomycin. Vancomycin resistance genes (vanA, vanB) and
biofilm formation (esp gene) were detected by amplifying the respective genes by PCR.
This study shows increase prevalence of Enterococci and VRE isolates as a cause of
urinary tract infection in our hospitals. It should be still careful of appropriate use of
antibiotics such as vancomycin. Detection and containment of VRE, a more targeted,
systematic approach is needed among patients.