The investigation was carried out at the laboratory of plant physiology, Bayreuth University, Germany on eight parents and their F; crosses for evaluation of trypsin inhibitor activity (TIA). Highly significant variations were noted for total protein percentage and trypsin inhibitor activity in parents and the obtained crosses at the two locations under study; Moshtohor and Nubaria. Tinova was the lowest variety for TIA while Giza 2 was the highest parent at both locations. The lowest content of Trypsin inhibitor and the highest content of protein as well as seed yield and its components were detected in the crosses; Rina Blanka X Tinova, Rina Blanka X Giza 429 and Triple white X Giza 2. The best crosses for all the studied characters especially trypsin inhibitor and protein content were Rina Blanka X Tina, Rina Blanka X Triple White and Rina Blanka X Tinova. The protein banding for trypsin inhibitor was found at 20-22 KDa for the studied parents at Nubaria location. The digested copolymerized SOS-gel electrophoresis emphasized a varied intensty of represented trypsin inhibitor bands for all parents and crosses at Nubaria location.