The investigation was carried out at the laboratory of plant physiology,
Bayreuth University, Germany on eight parents and their F; crosses for evaluation
of trypsin inhibitor activity (TIA). Highly significant variations were noted for
total protein percentage and trypsin inhibitor activity in parents and the obtained
crosses at the two locations under study; Moshtohor and Nubaria. Tinova was the
lowest variety for TIA while Giza 2 was the highest parent at both locations. The
lowest content of Trypsin inhibitor and the highest content of protein as well as
seed yield and its components were detected in the crosses; Rina Blanka X
Tinova, Rina Blanka X Giza 429 and Triple white X Giza 2. The best crosses for
all the studied characters especially trypsin inhibitor and protein content were
Rina Blanka X Tina, Rina Blanka X Triple White and Rina Blanka X Tinova. The
protein banding for trypsin inhibitor was found at 20-22 KDa for the studied
parents at Nubaria location. The digested copolymerized SOS-gel electrophoresis
emphasized a varied intensty of represented trypsin inhibitor bands for all parents
and crosses at Nubaria location.