Staff Members - Benha University
Liquid chromatographic method with ultraviolet absorbance detection for measurement of levamisole in chicken tissues, eggs and plasma
Journal of Chromatography B: Biomedical Sciences and applications • 2003
Publication Information
Authors
El-kholy, HM and Kemppainen, B
Keywords
Not Available
Journal
Journal of Chromatography B: Biomedical Sciences and applications
Publisher
Elsevier
Volume
796
Issue
2
Pages
371-377
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The development and validation of a high-performance liquid chromatographic and UV detection method was accomplished
for quantitative determination of levamisole in chicken tissues, eggs and plasma. The chromatographic separation was achieved
on Luna® 5m C18 column using a mobile phase of 0.2% acetic acid in water:methanol (50:50 (v/v)) and Pic B-7 low UV
reagent and the pH was adjusted to 7.31 with ammonium hydroxide and UV wavelength was 225 nm. Limits of quantification
were 0.025g/g for all tissues and 0.003g/ml for plasma. Limit of detection was 0.001g/g for tissues and plasma.
© 2003 Elsevier B.V. All rights reserved.
for quantitative determination of levamisole in chicken tissues, eggs and plasma. The chromatographic separation was achieved
on Luna® 5m C18 column using a mobile phase of 0.2% acetic acid in water:methanol (50:50 (v/v)) and Pic B-7 low UV
reagent and the pH was adjusted to 7.31 with ammonium hydroxide and UV wavelength was 225 nm. Limits of quantification
were 0.025g/g for all tissues and 0.003g/ml for plasma. Limit of detection was 0.001g/g for tissues and plasma.
© 2003 Elsevier B.V. All rights reserved.