| publication name | Liquid chromatographic method with ultraviolet absorbance detection for measurement of levamisole in chicken tissues, eggs and plasma |
|---|---|
| Authors | El-kholy, HM and Kemppainen, B |
| year | 2003 |
| keywords | |
| journal | Journal of Chromatography B: Biomedical Sciences and applications |
| volume | 796 |
| issue | 2 |
| pages | 371-377 |
| publisher | Elsevier |
| Local/International | International |
| Paper Link | http://www.sciencedirect.com/science/article/pii/S1570023203006597 |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
The development and validation of a high-performance liquid chromatographic and UV detection method was accomplished for quantitative determination of levamisole in chicken tissues, eggs and plasma. The chromatographic separation was achieved on Luna® 5m C18 column using a mobile phase of 0.2% acetic acid in water:methanol (50:50 (v/v)) and Pic B-7 low UV reagent and the pH was adjusted to 7.31 with ammonium hydroxide and UV wavelength was 225 nm. Limits of quantification were 0.025g/g for all tissues and 0.003g/ml for plasma. Limit of detection was 0.001g/g for tissues and plasma. © 2003 Elsevier B.V. All rights reserved.