The development and validation of a high-performance liquid chromatographic and UV detection method was accomplished
for quantitative determination of levamisole in chicken tissues, eggs and plasma. The chromatographic separation was achieved
on Luna® 5
m C18 column using a mobile phase of 0.2% acetic acid in water:methanol (50:50 (v/v)) and Pic B-7 low UV
reagent and the pH was adjusted to 7.31 with ammonium hydroxide and UV wavelength was 225 nm. Limits of quantification
were 0.025
g/g for all tissues and 0.003
g/ml for plasma. Limit of detection was 0.001
g/g for tissues and plasma.
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