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Developing a new genic SSR primer database in faba bean (Vicia faba L.)

Journal of Applied Genetics • 2021
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Publication Information
Authors Kareem A Khalifa, Shafik D Ibrahim, Hoda AS El-Garhy, Mahmoud MM Moustafa, FouadMaalouf, Alsamman M Alsamman, Aladdin Hamwieh, Achraf El Allali
Keywords Not Available
Journal Journal of Applied Genetics
Publisher Springer Berlin Heidelberg
Volume 62
Issue 3
Pages 373-387
publication.type Local
Paper Link Open Link
Supplementary Materials Not Available
Abstract

الوصف
The goal of this research was to develop a new genetic database of simple sequence repetition (SSR) primers for faba and classify them according to their target genes and respective biological processes. Approximately 75,605 and 148,196 previously published genomic and transcriptomic faba sequences, respectively, have been used to detect possible SSRs. The numbers of identified SSRs from each dataset were 25,502 and 12,319, respectively. The distribution of different repeat classes indicated that trinucleotides represent the largest number of repeat counts, followed by dinucleotides. The extracted genic SSR sequences were used to design 1091 polymerase chain reaction (PCR) primers, of which only 238 (21.8%) primers target genomic sequences and the other 853 PCR primers targeted transcriptomic sequences. The annotation of gene-targeted SSRs showed that approximately 897 genes