Developing a new genic SSR primer database in faba bean (Vicia faba L.)
Journal of Applied Genetics • 2021
Publication Information
Authors
Kareem A Khalifa, Shafik D Ibrahim, Hoda AS El-Garhy, Mahmoud MM Moustafa, FouadMaalouf, Alsamman M Alsamman, Aladdin Hamwieh, Achraf El Allali
Keywords
Not Available
Journal
Journal of Applied Genetics
Publisher
Springer Berlin Heidelberg
Volume
62
Issue
3
Pages
373-387
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
الوصف
The goal of this research was to develop a new genetic database of simple sequence repetition (SSR) primers for faba and classify them according to their target genes and respective biological processes. Approximately 75,605 and 148,196 previously published genomic and transcriptomic faba sequences, respectively, have been used to detect possible SSRs. The numbers of identified SSRs from each dataset were 25,502 and 12,319, respectively. The distribution of different repeat classes indicated that trinucleotides represent the largest number of repeat counts, followed by dinucleotides. The extracted genic SSR sequences were used to design 1091 polymerase chain reaction (PCR) primers, of which only 238 (21.8%) primers target genomic sequences and the other 853 PCR primers targeted transcriptomic sequences. The annotation of gene-targeted SSRs showed that approximately 897 genes
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