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publication name EVALUATION OF (PARP-1) EXPRESSION IN GASTRIC CARCINOMA BY IMMUNOHISTOCHEMISTRY AND QUANTITATIVE REAL-TIME PCR AND ITS RELATION TO HER2 STATUS.
Authors Marwa Said Abd Allaha , Heba mohammed Abd El Kariemb ,Heba Mohammed Rashada.
year 2020
keywords Key Words: PARP-1, HER2, gastric carcinoma, Immunohistochemistry, Quantitative Real-Time PCR.
journal The Medical Journal of Cairo University
volume Not Available
issue Not Available
pages Not Available
publisher Not Available
Local/International Local
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Abstract

Abstract: Background: PARP-1 overexpression has been identified in several malignancies. Few studies have handled the expression of PARP-1 protein in gastric carcinoma and its associations with outcome. This work aimed at exploring the significance of PARP-1 expression in gastric adenocarcinoma and correlation with HER2 status Methods: Quantitative expression of PARP-1 proteins was assayed by Immunohistochemistry in 40 cases of gastric adenocarcinoma. PARP-1 mRNA was moreover evaluated by Quantitative Real-Time Polymerase Chain Reaction. Results: The level of expression of mRNA expression level was significantly increased in 25 of 40 (62.5%) of gastric adenocarcinoma tissues compared with the corresponding adjacent non cancer tissues (P

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