Staff Members - Benha University
EVALUATION OF (PARP-1) EXPRESSION IN GASTRIC CARCINOMA BY IMMUNOHISTOCHEMISTRY AND QUANTITATIVE REAL-TIME PCR AND ITS RELATION TO HER2 STATUS.
The Medical Journal of Cairo University • 2020
Publication Information
Authors
Marwa Said Abd Allaha , Heba mohammed Abd El Kariemb ,Heba Mohammed Rashada.
Keywords
Key Words: PARP-1, HER2, gastric carcinoma, Immunohistochemistry, Quantitative Real-Time PCR.
Journal
The Medical Journal of Cairo University
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Abstract:
Background: PARP-1 overexpression has been identified in several malignancies. Few studies have handled the expression of PARP-1 protein in gastric carcinoma and its associations with outcome. This work aimed at exploring the significance of PARP-1 expression in gastric adenocarcinoma and correlation with HER2 status
Methods: Quantitative expression of PARP-1 proteins was assayed by Immunohistochemistry in 40 cases of gastric adenocarcinoma. PARP-1 mRNA was moreover evaluated by Quantitative Real-Time Polymerase Chain Reaction.
Results: The level of expression of mRNA expression level was significantly increased in 25 of 40 (62.5%) of gastric adenocarcinoma tissues compared with the corresponding adjacent non cancer tissues (P
Background: PARP-1 overexpression has been identified in several malignancies. Few studies have handled the expression of PARP-1 protein in gastric carcinoma and its associations with outcome. This work aimed at exploring the significance of PARP-1 expression in gastric adenocarcinoma and correlation with HER2 status
Methods: Quantitative expression of PARP-1 proteins was assayed by Immunohistochemistry in 40 cases of gastric adenocarcinoma. PARP-1 mRNA was moreover evaluated by Quantitative Real-Time Polymerase Chain Reaction.
Results: The level of expression of mRNA expression level was significantly increased in 25 of 40 (62.5%) of gastric adenocarcinoma tissues compared with the corresponding adjacent non cancer tissues (P