Salmonellosis is one of the most important zoonotic bacterial pathogen of food-borne infection all around the world.The present study was carried out to report the prevalence of the serotypes and genetic types of salmonella among broiler chickens,raw chickens meat and patients suffer from food poisoning signs in Toukh Egypt.Samples collected from (50) diarrheic broiler chicken,(50) raw frozen chickens meat and (30) diarrheic patients with food poisoning signs were bacteriologically and serologically processed for identification of Salmonella. Isolates were subjected to multiplex-PCR using specific Salmonella primers.The prevalence of Salmonella spp was 7(14%), 2(4%) and 3(10%) in chickens,raw chickens meat and patients respectively. TheSalmonella Isolates were serologically identified as 7(58.33%) and 5[41.66%] S.enteritidis and S.typhimurium respectively.The antigenic formula of serovar S.enteritidis has somatic antigen O: 1,9,12 and phase I (g,m) and phase II [1,7] flagellar antigen (H),While serovars S.typhimurium have the O: 1,4, (5),12and phase I and phase II.Multiplex-PCR yield similar diagnostic amplified DNA bands of molecular size marker at 250bp in tested S.enteritidis and 620bp in examined S.typhimurium,indicating the zoonotic potential of the organism and the role of chicken and chicken meat as sources in the epidemiology of the human salmonellosis