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Survival of vitrified water buffalo cumulus oocytes complex and their subsequent development in vitro

Asian Journal of Animal and Veterinary Advances • 2002
العودة
معلومات البحث
المؤلفون WA Khalil, Sh A Gabr, Sh M Shamiah, AMA El-Haif, AE Abdel-Khalek, M Asada, S Ishibashi, S Ikumi, Y Fukui, L Attanasio, L Boccia, G Vajta, M Kuwayama, G Campanile, H Bagis, HO Mercan, S Cetin, S Sekmen, D Boonkusol, T Faisaikarm, A Dinnyes, Y Kitiyanant, C
الكلمات المفتاحية Not Available
المجلة العلمية Asian Journal of Animal and Veterinary Advances
الناشر Not Available
المجلد Not Available
العدد Not Available
الصفحات Not Available
publication.type International
رابط البحث Not Available
المواد المرفقة Not Available
الملخص
This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p