| publication name | Survival of vitrified water buffalo cumulus oocytes complex and their subsequent development in vitro |
|---|---|
| Authors | WA Khalil, Sh A Gabr, Sh M Shamiah, AMA El-Haif, AE Abdel-Khalek, M Asada, S Ishibashi, S Ikumi, Y Fukui, L Attanasio, L Boccia, G Vajta, M Kuwayama, G Campanile, H Bagis, HO Mercan, S Cetin, S Sekmen, D Boonkusol, T Faisaikarm, A Dinnyes, Y Kitiyanant, C |
| year | 2002 |
| keywords | |
| journal | Asian Journal of Animal and Veterinary Advances |
| volume | Not Available |
| issue | Not Available |
| pages | Not Available |
| publisher | Not Available |
| Local/International | International |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p