Staff Members - Benha University
Survival of vitrified water buffalo cumulus oocytes complex and their subsequent development in vitro
Asian Journal of Animal and Veterinary Advances • 2002
Publication Information
Authors
WA Khalil, Sh A Gabr, Sh M Shamiah, AMA El-Haif, AE Abdel-Khalek, M Asada, S Ishibashi, S Ikumi, Y Fukui, L Attanasio, L Boccia, G Vajta, M Kuwayama, G Campanile, H Bagis, HO Mercan, S Cetin, S Sekmen, D Boonkusol, T Faisaikarm, A Dinnyes, Y Kitiyanant, C
Keywords
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Journal
Asian Journal of Animal and Veterinary Advances
Publisher
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Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p