Salmonellosis is one of the most common and widely distributed foodborne diseases, and it's presence in poultry and poultry products is a global public health problem. Therefore, the present study was conducted to isolate Salmonella from internal organs, fecal matter and eggs of freshly dead, diseased living and apparently healthy chickens and ducks in Dakahlia governorate (Egypt). A total of 400 samples were collected as follows: 280 chickens, 20 chicken eggs, 89 ducks and 11 duck eggs. The samples were examined bacteriologically and serotyped . Forty five samples (11.25%) were found to be positive for Salmonellosis. Ten strains were detected (S. Kentucky, S. Skansen, S. Typhimurium, S.Wingrove, S. Agona, S. Tananarive, S. Newport, S. Inganda, S. Enteritidis and S. Labadi). Untyped salmonellae were detected. The isolated Salmonella was sensitive to gentamycin, ciprofloxacin, colistin sulphate, doxycyclin hydrochloride and amoxicillin. Polymerase chain reaction( PCR) for detection common gene (invA) was applied to all isolated strains and showed positive amplification of 284 bp fragments.