Staff Members - Benha University
DETECTION OF COMMON (inv A) GENE IN SALMONELLAE ISOLATED FROM POULTRY USING POLYMERASE CHAIN RECTION TECHNIQUE
BVMJ • 2013
Publication Information
Authors
Ashraf, A. Abd El Tawwab a, Ahmed, M. Ammar b, Aisha, R. Ali c, Fatma, I. El Hofy a
and Mohammed, E. E. Sayed Ahmed d
Keywords
Salmonella, invA gene, PCR
Journal
BVMJ
Publisher
Faculty of Vet.Med. Benha Univ.
Volume
25
Issue
2
Pages
70-77
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Salmonellosis is one of the most common and widely distributed foodborne diseases, and it's presence
in poultry and poultry products is a global public health problem. Therefore, the present study was
conducted to isolate Salmonella from internal organs, fecal matter and eggs of freshly dead, diseased
living and apparently healthy chickens and ducks in Dakahlia governorate (Egypt). A total of 400
samples were collected as follows: 280 chickens, 20 chicken eggs, 89 ducks and 11 duck eggs. The
samples were examined bacteriologically and serotyped . Forty five samples (11.25%) were found to be
positive for Salmonellosis. Ten strains were detected (S. Kentucky, S. Skansen, S. Typhimurium,
S.Wingrove, S. Agona, S. Tananarive, S. Newport, S. Inganda, S. Enteritidis and S. Labadi). Untyped
salmonellae were detected. The isolated Salmonella was sensitive to gentamycin, ciprofloxacin, colistin
sulphate, doxycyclin hydrochloride and amoxicillin. Polymerase chain reaction( PCR) for detection
common gene (invA) was applied to all isolated strains and showed positive amplification of 284 bp
fragments.
in poultry and poultry products is a global public health problem. Therefore, the present study was
conducted to isolate Salmonella from internal organs, fecal matter and eggs of freshly dead, diseased
living and apparently healthy chickens and ducks in Dakahlia governorate (Egypt). A total of 400
samples were collected as follows: 280 chickens, 20 chicken eggs, 89 ducks and 11 duck eggs. The
samples were examined bacteriologically and serotyped . Forty five samples (11.25%) were found to be
positive for Salmonellosis. Ten strains were detected (S. Kentucky, S. Skansen, S. Typhimurium,
S.Wingrove, S. Agona, S. Tananarive, S. Newport, S. Inganda, S. Enteritidis and S. Labadi). Untyped
salmonellae were detected. The isolated Salmonella was sensitive to gentamycin, ciprofloxacin, colistin
sulphate, doxycyclin hydrochloride and amoxicillin. Polymerase chain reaction( PCR) for detection
common gene (invA) was applied to all isolated strains and showed positive amplification of 284 bp
fragments.