أعضاء هيئة التدريس - جامعة بنها
Isolation and Molecular Characterization of Lumpy Skin Disease Virus from Kalubia Governorate
• 2009
معلومات البحث
المؤلفون
G.F. El-Bagoury, E.M. El-Nahas, A.S. El-Habbaa and E.T. Abdel-Nour
الكلمات المفتاحية
Not Available
المجلة العلمية
Not Available
الناشر
Not Available
المجلد
Not Available
العدد
Not Available
الصفحات
Not Available
publication.type
Local
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected cattle at Kalubia governorate. The virus was isolated on chorio-allantoic membrane (CAM) of embryonated
chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific antisera against LSDV. Further electron microscopic (EM) examination and
polymerase chain reaction (PCR) for putative fusion protein gene have been adopted. Nucleotide sequencing of fusion protein gene indicated that the isolate was genetically related to the reference LSDV Neethling
vaccinal strain.
chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific antisera against LSDV. Further electron microscopic (EM) examination and
polymerase chain reaction (PCR) for putative fusion protein gene have been adopted. Nucleotide sequencing of fusion protein gene indicated that the isolate was genetically related to the reference LSDV Neethling
vaccinal strain.