Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected cattle at Kalubia governorate. The virus was isolated on chorio-allantoic membrane (CAM) of embryonated chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific antisera against LSDV. Further electron microscopic (EM) examination and polymerase chain reaction (PCR) for putative fusion protein gene have been adopted. Nucleotide sequencing of fusion protein gene indicated that the isolate was genetically related to the reference LSDV Neethling vaccinal strain.