Staff Members - Benha University
Isolation and Molecular Characterization of Lumpy Skin Disease Virus from Kalubia Governorate
• 2009
Publication Information
Authors
G.F. El-Bagoury, E.M. El-Nahas, A.S. El-Habbaa and E.T. Abdel-Nour
Keywords
Not Available
Journal
Not Available
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected cattle at Kalubia governorate. The virus was isolated on chorio-allantoic membrane (CAM) of embryonated
chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific antisera against LSDV. Further electron microscopic (EM) examination and
polymerase chain reaction (PCR) for putative fusion protein gene have been adopted. Nucleotide sequencing of fusion protein gene indicated that the isolate was genetically related to the reference LSDV Neethling
vaccinal strain.
chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific antisera against LSDV. Further electron microscopic (EM) examination and
polymerase chain reaction (PCR) for putative fusion protein gene have been adopted. Nucleotide sequencing of fusion protein gene indicated that the isolate was genetically related to the reference LSDV Neethling
vaccinal strain.