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publication name Molecular studies regarding to virulence factors of Streptococcus species isolated from raw milk
Authors 1 Ashraf, A. AbdEL-Tawab, ² Nahla, A. Abou El-Roos, 1 Fatma, I. El- Hofy and 3 Hassnaa, E. Abdullah
year 2017
keywords Streptococcus spp., Raw milk, PCR
journal BVMJ
volume Not Available
issue Not Available
pages Not Available
publisher Not Available
Local/International International
Paper Link Not Available
Full paper download
Supplementary materials Not Available
Abstract

The present study was performed on a total of 124 milk samples from small scale producers, farmers and markets at El-Menofyia Governorate.The prevalence of Streptococcus species in the examined samples was (65.3%),where S. agalctiae, S. dysgalactiae, S. uberis, S. pyogenes and S. pneumoniae were 28.2%, 11.3%,16.1%, 8.9% and 0.8%; respectively. The antibiogram for Streptococcus spp. revealed that vancomycin and erythromycin were the most proper antibiotics with the highest in vitro efficiency against isolated Streptococcus spp., but were resistant to cefatriaxone and chlormphenicol.Additionally, S. agalactiae and S.dysgalactiae were sensitive to penicillin, ofloxacin ;respctively.But S.uberis was sensitive to amoxicillin and clindamycin. By using PCR, virulence gene hyalurinidase (hyl) was detected in 25% of S. agalactiae, while monokine induced by IFN-gamma (mig) gene was detected in 100% of S. dysgalactiae. Also plasminogen activator(pauA) gene was detected in 100% of S. uberis isolates

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