Staff Members - Benha University
Molecular studies regarding to virulence factors of Streptococcus species isolated from raw milk
BVMJ • 2017
Publication Information
Authors
1
Ashraf, A. AbdEL-Tawab, ² Nahla, A. Abou El-Roos,
1
Fatma, I. El-
Hofy and
3
Hassnaa, E. Abdullah
Keywords
Streptococcus spp., Raw milk, PCR
Journal
BVMJ
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The present study was performed on a total of 124 milk samples from
small scale producers, farmers and markets at El-Menofyia
Governorate.The prevalence of Streptococcus species in the examined
samples was (65.3%),where S. agalctiae, S. dysgalactiae, S. uberis, S.
pyogenes and S. pneumoniae were 28.2%, 11.3%,16.1%, 8.9% and
0.8%; respectively. The antibiogram for Streptococcus spp. revealed
that vancomycin and erythromycin were the most proper antibiotics
with the highest in vitro efficiency against isolated Streptococcus
spp., but were resistant to cefatriaxone and
chlormphenicol.Additionally, S. agalactiae and S.dysgalactiae were
sensitive to penicillin, ofloxacin ;respctively.But S.uberis was
sensitive to amoxicillin and clindamycin. By using PCR, virulence
gene hyalurinidase (hyl) was detected in 25% of S. agalactiae, while
monokine induced by IFN-gamma (mig) gene was detected in 100%
of S. dysgalactiae. Also plasminogen activator(pauA) gene was
detected in 100% of S. uberis isolates
small scale producers, farmers and markets at El-Menofyia
Governorate.The prevalence of Streptococcus species in the examined
samples was (65.3%),where S. agalctiae, S. dysgalactiae, S. uberis, S.
pyogenes and S. pneumoniae were 28.2%, 11.3%,16.1%, 8.9% and
0.8%; respectively. The antibiogram for Streptococcus spp. revealed
that vancomycin and erythromycin were the most proper antibiotics
with the highest in vitro efficiency against isolated Streptococcus
spp., but were resistant to cefatriaxone and
chlormphenicol.Additionally, S. agalactiae and S.dysgalactiae were
sensitive to penicillin, ofloxacin ;respctively.But S.uberis was
sensitive to amoxicillin and clindamycin. By using PCR, virulence
gene hyalurinidase (hyl) was detected in 25% of S. agalactiae, while
monokine induced by IFN-gamma (mig) gene was detected in 100%
of S. dysgalactiae. Also plasminogen activator(pauA) gene was
detected in 100% of S. uberis isolates