Cumcumber Mosaic Viruss (CMV) was isolated from naturally infected Banana, Geranium and Gladiolus and exhibited different external symptoms. The three CMV isolates were transmitted by sap inoculation with 6, 5 and 5 out of 10 inoculated N. glutinosa plants as well as by aphids with 6, 5 and 4 out of 10 inoculated Cucumber plants, respectively. These isolates exhibited different morphological characters of single local lesion on Chenopodium amaranticolor and had different sap stability (TIP, DEP and LIV) in vitro. The differential host reactions were differed among these isolates in incubation period and external symptoms; the amplified cp-gene RNA of confirm the diversity of CMV isolates. It was proved that CMV could involve variation of infection due to one more than CMV strains. It has been declared that the complementary role of biological diagnosis addition, in addition to molecular diagnosis to have a complete and clear image for viral etiology.