Staff Members - Benha University
Telomere length and signal joint T-cell receptor rearrangement excision circles as biomarkers for chronological age estimation
Biomarkers • 2021
Publication Information
Authors
Mostafa Ali Elmadawy , Omnia A. Abdullah , Walaa Bayoumie El Gazzar ,
Enas Sebaey Ahmad , Seham Gouda Ameen & Afaf Abdelkader
Keywords
Chronological age; telomere
length; sjTRECs; quantitative
real-time PCR;
southern blotting
Journal
Biomarkers
Publisher
Taylor & Francis
Volume
26
Issue
2
Pages
Not Available
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Background: Chronological age estimation is a challenging marker in the field of forensic medicine.
The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision
circles (sjTRECs) quantification and telomere length measurement as methods for estimating
chronological age.
Methods: Telomere length was estimated in the DNA derived from the buccal cells through estimating
the telomeric restriction fragment (TRF) length using TeloTTAGGG Telomere Length Assay while
the sjTRECs quantification was carried out on DNA isolated from the blood samples using qPCR.
Results: The TRF length was shortened with increased age (r ¼ 0.722, p
The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision
circles (sjTRECs) quantification and telomere length measurement as methods for estimating
chronological age.
Methods: Telomere length was estimated in the DNA derived from the buccal cells through estimating
the telomeric restriction fragment (TRF) length using TeloTTAGGG Telomere Length Assay while
the sjTRECs quantification was carried out on DNA isolated from the blood samples using qPCR.
Results: The TRF length was shortened with increased age (r ¼ 0.722, p