أعضاء هيئة التدريس - جامعة بنها
Telomere length and signal joint T-cell receptor rearrangement excision circles as biomarkers for chronological age estimation
Biomarkers • 2021
معلومات البحث
المؤلفون
Mostafa Ali Elmadawy , Omnia A. Abdullah , Walaa Bayoumie El Gazzar ,
Enas Sebaey Ahmad , Seham Gouda Ameen & Afaf Abdelkader
الكلمات المفتاحية
Chronological age; telomere
length; sjTRECs; quantitative
real-time PCR;
southern blotting
المجلة العلمية
Biomarkers
الناشر
Taylor & Francis
المجلد
26
العدد
2
الصفحات
Not Available
publication.type
International
رابط البحث
Open Link
المواد المرفقة
Not Available
الملخص
Background: Chronological age estimation is a challenging marker in the field of forensic medicine.
The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision
circles (sjTRECs) quantification and telomere length measurement as methods for estimating
chronological age.
Methods: Telomere length was estimated in the DNA derived from the buccal cells through estimating
the telomeric restriction fragment (TRF) length using TeloTTAGGG Telomere Length Assay while
the sjTRECs quantification was carried out on DNA isolated from the blood samples using qPCR.
Results: The TRF length was shortened with increased age (r ¼ 0.722, p
The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision
circles (sjTRECs) quantification and telomere length measurement as methods for estimating
chronological age.
Methods: Telomere length was estimated in the DNA derived from the buccal cells through estimating
the telomeric restriction fragment (TRF) length using TeloTTAGGG Telomere Length Assay while
the sjTRECs quantification was carried out on DNA isolated from the blood samples using qPCR.
Results: The TRF length was shortened with increased age (r ¼ 0.722, p