Staff Members - Benha University
Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes
Iranian Journal of Veterinary Research, Shiraz University • 2016
Publication Information
Authors
Mahmoud, K. Gh. M.; El-Sokary, M. M. M.; Kandiel, M. M. M.;
Abou El-Roos, M. E. A. and Sosa, G. M. S
Keywords
Buffalo oocytes, Cysteamine, Meiotic maturation, Vitrification
Journal
Iranian Journal of Veterinary Research, Shiraz University
Publisher
Not Available
Volume
17
Issue
3
Pages
165-170
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with
compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium
with no cysteamine (control) or 50 μM cysteamine (treated). Oocytes were vitrified in vitrification solution 1 (VS1): 1.5 M ethylene
glycol (EG) + 1.5 M dimethyl sulfoxide (DMSO) for 45 s (step one). After this initial exposure, oocytes were transferred to VS2: 3
M EG + 3 M DMSO in a holding medium for 25 s (step two). After warming, oocytes were evaluated morphologically and then
cultured for a further 2 h in the cysteamine-supplemented or control maturation media. The oocytes were evaluated morphologically,
stained with trypan blue for viability evaluation. The maturation rate of oocytes was higher (P
compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium
with no cysteamine (control) or 50 μM cysteamine (treated). Oocytes were vitrified in vitrification solution 1 (VS1): 1.5 M ethylene
glycol (EG) + 1.5 M dimethyl sulfoxide (DMSO) for 45 s (step one). After this initial exposure, oocytes were transferred to VS2: 3
M EG + 3 M DMSO in a holding medium for 25 s (step two). After warming, oocytes were evaluated morphologically and then
cultured for a further 2 h in the cysteamine-supplemented or control maturation media. The oocytes were evaluated morphologically,
stained with trypan blue for viability evaluation. The maturation rate of oocytes was higher (P