buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with
compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium
with no cysteamine (control) or 50 μM cysteamine (treated). Oocytes were vitrified in vitrification solution 1 (VS1): 1.5 M ethylene
glycol (EG) + 1.5 M dimethyl sulfoxide (DMSO) for 45 s (step one). After this initial exposure, oocytes were transferred to VS2: 3
M EG + 3 M DMSO in a holding medium for 25 s (step two). After warming, oocytes were evaluated morphologically and then
cultured for a further 2 h in the cysteamine-supplemented or control maturation media. The oocytes were evaluated morphologically,
stained with trypan blue for viability evaluation. The maturation rate of oocytes was higher (P