Staff Members - Benha University
Assessment of inhibitory activity of hydrolyzed camel whey protein concentrate and its peptides against some fungi
American Journal of Food Science and Nutrition (AJFSN) • 2023
Publication Information
Authors
Eman Abd El Samei Bakri Nafei, ,, Ekbal Mohammed Adel Ibrahim, Hend Ahmed Elbarbary
Keywords
Camel´s WPC, enzymatic hydrolysis, peptide fractions, antifungal potancy
Journal
American Journal of Food Science and Nutrition (AJFSN)
Publisher
SDIP press
Volume
Vol - 03
Issue
Issue - 01
Pages
297-315
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The objective of this study was to investigate the antifungal activity of different
concentrations (5, 10 and 20 mg/ml) of camel whey protein concentrate (WPC) and its hydrolysates against
Candida albicans, Asperigillus fumigatus, Asperigillus niger and Asperigillus flavusThen, the strongest
antifungal hydrolysate was further fractionated on fast performance liquid chromatography (FPLC) to
separate the potent peptide subfractions at concentration of 1.45 mg/ml. The results revealed that the pepsintrypsin (P-T) hydrolysate (20 mg/ml) showed the strongest antifungal activity, followed by 20 mg/ml of both
unhydrolyzed WPC and pepsin (P) hydrolysate against C.albicans, A.fumigatus, A.niger and A.flavus,
respectively.However all tested conentrations of trypsin (T) hydrolysate had the lowest activity against all
strains.Thus, the potent P-T hydrolysate was subjected to further fractionation and the potent lower molecular
weight peptide subfractions (SFs) (< 20kDa) were SF18 against A.niger, SF14 against A.fumigatus and
A.flavus, repectively and SF21 against C.albicans.Camel´s WPC, its hydrolysates and the potent peptide
subfractions (SF18 and SF14) had more hydrophobic amino acids, however SF21 had more hydrophilic
amino acids. Consequently, it′s concluded that P-T camel´s WPC hydrolysate can be applied as a natural
effective antifungal agent
concentrations (5, 10 and 20 mg/ml) of camel whey protein concentrate (WPC) and its hydrolysates against
Candida albicans, Asperigillus fumigatus, Asperigillus niger and Asperigillus flavusThen, the strongest
antifungal hydrolysate was further fractionated on fast performance liquid chromatography (FPLC) to
separate the potent peptide subfractions at concentration of 1.45 mg/ml. The results revealed that the pepsintrypsin (P-T) hydrolysate (20 mg/ml) showed the strongest antifungal activity, followed by 20 mg/ml of both
unhydrolyzed WPC and pepsin (P) hydrolysate against C.albicans, A.fumigatus, A.niger and A.flavus,
respectively.However all tested conentrations of trypsin (T) hydrolysate had the lowest activity against all
strains.Thus, the potent P-T hydrolysate was subjected to further fractionation and the potent lower molecular
weight peptide subfractions (SFs) (< 20kDa) were SF18 against A.niger, SF14 against A.fumigatus and
A.flavus, repectively and SF21 against C.albicans.Camel´s WPC, its hydrolysates and the potent peptide
subfractions (SF18 and SF14) had more hydrophobic amino acids, however SF21 had more hydrophilic
amino acids. Consequently, it′s concluded that P-T camel´s WPC hydrolysate can be applied as a natural
effective antifungal agent