Staff Members - Benha University
Comparison of different genotypes of fennel (Foeniculum vulgare Mill.) in terms of chemical compounds extracted from seeds and in the callus induced from tissue culture
Current Science International • 2014
Publication Information
Authors
El Nagar, M. M. and Mekawi, E. M.
Keywords
Not Available
Journal
Current Science International
Publisher
Not Available
Volume
3
Issue
4
Pages
445-453
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A laboratory study was conducted to evaluate the essential oil composition, total phenolic compound,
total flavonoid compound and the antioxidant activity in seed and callus induced from tissue culture of two
fennel genotypes. The influence of genotype, explants type and growth regulators concentrations on callus
induction was investigated.
Results strongly showed that there were significant differences between two fennel genotypes.
Generally the highest values of all parameters were obtained from Balady genotype. Callus was induced from
two explants type on MS medium containing different combinations of 2,4-D and Kinetin.
The essential oil characterization by GC revealed that seed oil had the highest amount of trans-Anethol
of two genotypes. On the other hand, callus oil from Balady genotype had the highest amount of α-Pinene while
Romanesco genotype had the highest amount of p-Cymene. Balady genotype had the highest amount of total
phenolic and flavonoid content in seed as well as callus induced from tissue culture compared with Romanesco
genotype. The antioxidant activities of both genotypes were evaluated and Balady genotype showed the highest
DPPH_ scavenging activity expressed as IC50 compared with Romanesco genotype.
total flavonoid compound and the antioxidant activity in seed and callus induced from tissue culture of two
fennel genotypes. The influence of genotype, explants type and growth regulators concentrations on callus
induction was investigated.
Results strongly showed that there were significant differences between two fennel genotypes.
Generally the highest values of all parameters were obtained from Balady genotype. Callus was induced from
two explants type on MS medium containing different combinations of 2,4-D and Kinetin.
The essential oil characterization by GC revealed that seed oil had the highest amount of trans-Anethol
of two genotypes. On the other hand, callus oil from Balady genotype had the highest amount of α-Pinene while
Romanesco genotype had the highest amount of p-Cymene. Balady genotype had the highest amount of total
phenolic and flavonoid content in seed as well as callus induced from tissue culture compared with Romanesco
genotype. The antioxidant activities of both genotypes were evaluated and Balady genotype showed the highest
DPPH_ scavenging activity expressed as IC50 compared with Romanesco genotype.