The higher bioavailability, lower toxicity, and lower potential environmental risks of zinc oxide nanoparticles (ZnO NPs) compared to traditional sources of zinc (Zn) encouraged researchers to use it in animal diets. The current study aimed to investigate the effects of dietary low ZnO NPs levels supplementation on the meat yield of Shami goats using ultrasound examination. A total of thirty healthy female Shami goats with an average body weight of 38.43 ±1.94 kg at 2–3 years old were randomly distributed into three equal groups (10 goats/group). The first group was fed a basal diet (control), while the second and third groups were fed a basal diet supplemented with 11.25 mg/kg (T1) and 22.50 mg/kg (T2) dry matter (DM) of ZnO NPs, respectively, for 12 weeks. Longissimus dorsi muscle ultrasound evaluation at both the thoracic and lumbar regions revealed denser transverse process-related acoustic shadowing (TS), rib-related acoustic shadowing (RS), and muscular shadowing (MS) in goats supplemented with ZnO NPs, T1 and T2, compared to goats fed a control diet. Furthermore, ultrasound measurements of the thoracic and lumbar longissimus dorsi regions of goats supplemented with ZnO NPs at both levels revealed greater fat thickness, depth, perimeter, and area compared to animals fed a control diet (p