Background: Talent management has been on the agenda of many health care organizations because of the belief in its importance in achieving organizational excellence and make their personnel more affiliated to achieve organizational objectives. Aim: The study aims to evaluate the effect of educational program about talent management for nursing managers on their job affiliation and organizational excellence. Research Design: A quasi-experimental research design was utilized to carry out the current study. Setting: The study was conducted in all (48) units at Benha University Hospital Qaluobia Governate, Egypt. Subjects: all (52) nursing managers that include (1) Nursing Director, (3) Supervisors, and (48) head nurses. Tools: Four tools were used in this study; First: Talent Management Knowledge Questionnaire, Second: Talent Management activities Questionnaire, Third: Job Affiliation Questionnaire and the fourth tool: Organizational Excellence Questionnaire. Results: The study findings revealed that there were highly statistical significant improvement in the nursing managers' levels of talent management knowledge and activities, job affiliation and organizational excellence after intervention both immediately post and follow up the program. Conclusion: The study concluded that there were statistically significant positive correlation among nurse managers' talent management knowledge and activities scores with their job affiliation and organizational excellence scores. Recommendation: Establishing bases for recruitment and selection in hospital that rely on the criteria of talents acquisition.