| publication name | GROWTH CHARACTERISTICS OF LOW PATHOGENIC STRAIN OF AVIAN INFLUENZA VIRUS (H5N2) IN DIFFERENT CELL CULTURES |
|---|---|
| Authors | Zeinab, R. A. Abo Ezz, El-Bagoury, G.F., Khodier, M.H. and Sharawi, S.S.A. |
| year | 2013 |
| keywords | influenza , cell culture , propagation |
| journal | Benha Veterinary Medical Journal |
| volume | 25 |
| issue | 1 |
| pages | 210-217 |
| publisher | Not Available |
| Local/International | Local |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
A low pathogenic strain of avian influenza virus (H5N2) was successfully propagated in both primary chicken embryo fibroblast (CEF) cells and cell line of baby hamster kidney (BHK-21), African green monkey kidney (Vero) and Madine Darby canine kidney (MDCK) cells through ten successive passages; the virus induced cytopathic effect (CPE) from the 1st passage characterized by cell rounding, elongation and cell disintegration. The most suitable cell cultures for virus propagation were MDCK and BHK-21 cells that gave the highest titer 107.5 and 107 TCID50/ml respectively at the 10th passage while the highest titer for Vero and CEF cells were 105.5 and 106.5 TCID50/ml respectively at the 10th passage. H5N2 virus was cell free virus with higher titer comparing to the cell associated virus in both BHK-21 and MDCK cell line