The present work aimed to study the effects of some preservatives materials treatments (pulsing and holding solutions) and cold storage for different durations each alone, as well as their combination ones on the postharvest characters, water relation characters, bacterial counts and chemical constituents of chrysanthemum Dendranthema grandiflorum kitam cut flower spikes . cv. "White Zambla". The present work studied three factors and the interactions between them. The first factor was pulsing solution treatments as (D.W) for 24 hours (control treatment),silver thiosulphate (STS) at 0.4mM for 30minutes then 10ppm benzyladenine (BA) for 24 hours, silver thiosulphate (STS) at 0.4mM for 30minutes then 20ppm Gibberellic acid (GA3) for 24 hours, [(GA3) at 20ppm)+ Aminooxy acetic acid (AOA) at 4mM] for 24 hours, silver thiosulphate (STS) at 0.4mM for 30minutes then pulsed in [(BA) at 10ppm + (GA3) at 20ppm + (AOA) at 4mM] for 24 hours. Whereas, the second factor was storage period treatments, chrysanthemum cut flower spikes were stored at 2 ± 1°C for different days (0-time, 7, 14 and 21 days). While, the third factor was holding solution treatments as D.W(control treatment) , sucrose (S) 2% + Citric acid (CA) at 100ppm + Silver nitrate (AgNO3) at 25 ppm and Sucrose (S) at 2% + 8-hydroxy-quinoline sulphate (8-HQS) at 100ppm + Citric acid (CA) at 100ppm besides to the interaction treatments between them. In addition, the data recorded were postharvest characters, water relation characters, average of bacterial counts in vase solution and some chemical constituents . Generally, the higher quality and the longest vase life of Chrysanthemum (Dendranthema grandiflorum kitam.) cut flower spikes were obtained by using the interaction treatments between pulsing solutions of silver thiosulphate (STS) at 0.4mM for 30minutes then pulsed in [(BA) at 10ppm + (GA3) at 20ppm + (AOA) at 4mM] for 24 hours , storage periods at 2±1°C for (0-time and 7days) and holding solution which contained sucrose at 2% + 8-hydroxy-quinolene sulfate at 100 mg/L + citric acid at 100 mg/L compared to the other ones under study.