Staff Members - Benha University
Relationship between sperm progressive motility and DNA integrity in fertile and infertile men
• 2018
Publication Information
Authors
Salah Elbashir a, Yasmin Magdi b,⇑, Ayman Rashed c, Mohamed Ahmed Ibrahim d, Yehia Edris d,
Ahmed Mostafa Abdelaziz
Keywords
DNA fragmentation
Progressive motility
Male infertility
Asthenozoospermia
Semen analysis
Modified sperm chromatin dispersion test
Semen parameters
Journal
Not Available
Publisher
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Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
Background: Progressive sperm motility was found to be predictive for in vitro and in vivo fertilization.
There has been an increase in the literature of studies investigating whether DNA fragmentation could
be associated with other semen parameters; however, few reports focused on the relationship between
sperm DNA fragmentation and progressive sperm motility.
Purpose: We purposed here to determine the relationship between DNA fragmentation level and progressive
sperm motility in different groups of infertile asthenozoospermic patients as well as in healthy men
of proven fertility.
Study design: Retrospective study.
Setting: Andrology Laboratory of the Assisted Reproductive Technologies (ART) department of Elite fertility
and gynecology center, Cairo, Egypt.
Patients and methods: Semen samples were collected and examined after liquefaction for 20 min at 37 C
from 182 patients. Patient were then classified as asthenozoospermic [(Mild asthenozoospermia; PR
(progressive sperm motility) = 30–20%, n = 58), (Moderate asthenozoospermia; PR = 20–10%, n = 68)
and (Severe asthenozoospermia; PR < 10%, n = 56)] and 32 fertile healthy men as a control.
Outcome results: Fertile healthy men showed lower sperm DNA fragmentation levels as compared with
asthenozoospermic infertile men. There was a significant negative correlation of sperm DNA fragmentation
using the modified sperm chromatin dispersion (SCD) test with motility (r = 0.319; P < .001) and
progressive motility (r = 0.474; P < .001).
Conclusion: Overall, our data suggest that sperm DNA damage is strongly associated with both type and
percentage of motility.
There has been an increase in the literature of studies investigating whether DNA fragmentation could
be associated with other semen parameters; however, few reports focused on the relationship between
sperm DNA fragmentation and progressive sperm motility.
Purpose: We purposed here to determine the relationship between DNA fragmentation level and progressive
sperm motility in different groups of infertile asthenozoospermic patients as well as in healthy men
of proven fertility.
Study design: Retrospective study.
Setting: Andrology Laboratory of the Assisted Reproductive Technologies (ART) department of Elite fertility
and gynecology center, Cairo, Egypt.
Patients and methods: Semen samples were collected and examined after liquefaction for 20 min at 37 C
from 182 patients. Patient were then classified as asthenozoospermic [(Mild asthenozoospermia; PR
(progressive sperm motility) = 30–20%, n = 58), (Moderate asthenozoospermia; PR = 20–10%, n = 68)
and (Severe asthenozoospermia; PR < 10%, n = 56)] and 32 fertile healthy men as a control.
Outcome results: Fertile healthy men showed lower sperm DNA fragmentation levels as compared with
asthenozoospermic infertile men. There was a significant negative correlation of sperm DNA fragmentation
using the modified sperm chromatin dispersion (SCD) test with motility (r = 0.319; P < .001) and
progressive motility (r = 0.474; P < .001).
Conclusion: Overall, our data suggest that sperm DNA damage is strongly associated with both type and
percentage of motility.