Staff Members - Benha University
Employment of Tissue Culture Techniques in Improvement Propagation of Paulownia Tomentosa Plant
J. Plant Production, Mansoura Univ. • 2016
Publication Information
Authors
Ghatas, Y.A.A.
Keywords
In vitro culture, Paulownia tomentosa, woody plants.
Journal
J. Plant Production, Mansoura Univ.
Publisher
J. Plant Production, Mansoura Univ.
Volume
7
Issue
6
Pages
619 - 625
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Different media and explant types, anti-oxidant pre-treatments, additives, cytokinin types &concentrations as well as
medium strength, GA3 concentrations, auxin types &concentrations were studied to find out the best protocol of in vitro propagation of Paulownia tomentosa. The results indicated that culturing of pre-treated shoot tips with anti-oxidant solution on modified Murashige&Skoog medium supplemented with PVP and activated charcoal as anti-oxidants, as well as adenine sulphate and coconut milk as additives maximized survival percentage and improved explant development. Also, using of 2mg/ L BAP increased proliferation. Addition of 1.0 mg/ L GA3 to half strength medium maximized shoot length. Moreover, addition of1.0 IBA to the culture medium encouraged the highest shoot length and number of roots.
medium strength, GA3 concentrations, auxin types &concentrations were studied to find out the best protocol of in vitro propagation of Paulownia tomentosa. The results indicated that culturing of pre-treated shoot tips with anti-oxidant solution on modified Murashige&Skoog medium supplemented with PVP and activated charcoal as anti-oxidants, as well as adenine sulphate and coconut milk as additives maximized survival percentage and improved explant development. Also, using of 2mg/ L BAP increased proliferation. Addition of 1.0 mg/ L GA3 to half strength medium maximized shoot length. Moreover, addition of1.0 IBA to the culture medium encouraged the highest shoot length and number of roots.