Different media and explant types, anti-oxidant pre-treatments, additives, cytokinin types &concentrations as well as medium strength, GA3 concentrations, auxin types &concentrations were studied to find out the best protocol of in vitro propagation of Paulownia tomentosa. The results indicated that culturing of pre-treated shoot tips with anti-oxidant solution on modified Murashige&Skoog medium supplemented with PVP and activated charcoal as anti-oxidants, as well as adenine sulphate and coconut milk as additives maximized survival percentage and improved explant development. Also, using of 2mg/ L BAP increased proliferation. Addition of 1.0 mg/ L GA3 to half strength medium maximized shoot length. Moreover, addition of1.0 IBA to the culture medium encouraged the highest shoot length and number of roots.