Purpose: This study aimed to evaluate the diagnostic yield of contrast-enhanced color Doppler ultrasonography (CECD-US) as a diagnostic modality for differentiation between retinal detachment (RD) and vitreous membrane (VM) in patients with vitreous opacity. Methods: The study included 26 patients presented by vitreous opacitites in one eye (26 eyes) after clinical evaluations, all patients underwent US examination. During all Doppler studies the diagnostic criterion for RD and VM was whether or not color signals were visualize din the membranous structure. Results: There were 12 eyes (46.2%) with VM, 6 eyes (23.1 %) had VM and RD, 6 eyes (23.1%) had partial RD and 2 eyes (7.6%) had complete RD. examined eyes were categorized as 12 eyes (46.2%) with VM and 14 eyes (53.8%) with RD. The diagnoses were confirmed by surgery in 23 patients, and by clinical follow-up in one with RD and two with VM. Gray scale US examination showed 3 false positives and 3 false positives with 100% specificity and positive predictive value (PPV); however, CD without contrast falsely excluded RD in only one eye with a sensitivity rate of 92.9%, negative predictive value (NPV) of 92.3% and accuracy of diagnosis of 96.2%. There was a significant increase of diagnostic validity characters of CECD-US in comparison to CD-US and gray-scale US, (X2 = 3.471, p