The point of this investigation was to decide the hereditary inconstancy and break down the
connection between four faba bean cultivars and six promising lines utilizing inter simple sequence repeats
(ISSR) markers. Faba bean genotypes were gathered by their resistance to the bean yellow mosaic virus
infection. With the acquired band registration, a paired data matrix was built to perform the comparing
statistical analysis. The used ISSR markers created 148 fixed and repeatable bands, of which 75 were
polymorphic. The values of resolution power (Rp), polymorphic information content (PIC), and marker index
(MI), uncovered that primer ISSR-14 was the most effective to break down hereditary changeability with
estimations of 5.8, 0.80, and 6.40, respectively, followed by primers ISSR-6 and ISSR-18. Genetic distances
swayed somewhere in the range of 0.78 and 0.91, and checked the groupings saw in the dendrogram, which
demonstrates high change at the degree of DNA among the investigated genotypes, watching six characterized
gatherings as indicated by UPGMA examination. In the examination of fundamental segments, the enrolled
groupings were dictated by the source of the origin of the gathering. The utilization of ISSR markers was
effective to describe at the degree of DNA the evaluated bean genotypes, showing the presence of inconstancy,
the recognized differentiating promotions can be used in hereditary improvement projects planned for settling
the requirements of the producers.