Objective: Comparing different effects of supplementation of Ham’s F10 media with vitamin E, Baker’s Yeast (Saccharomyces cerevisiae) extraction and different concentrations of vitamin C on sperm parameters. Patients and methods: 40 ejaculated semen samples of infertile men were obtained. Each semen sample was divided into 6 equal fractions. A Control group (activated with Ham’s F10 only). 5 study groups were activated with Ham’s F10 plus: Group 1: Vit E (conc. 2 mg/ml Ham’s F10), groups 2,3,4 (Vit C1, Vit C2 and Vit C3) (conc. 0.02, 0.04, 0.06 mg/ml Ham’s F10, respectively) ), Group 6: Yeast ( 20 mg/ml Ham’s F10). Results: Sperm total motility, progressive motility, vitality and oxidative stress were significantly increased in treated groups than the control group. The results showed that Vit. E has the best results (72%, 45%, 95%& 0) with comparable results with Yeast group (66%, 39%, 95%& 0) and the best result for Vit C was with conc. (0.06 mg/ml) in group Vit C3(65%,39%, 95%& 0), respectively. Conclusions: Vit. C, E and yeast, all have beneficial effects on semen parameters. Vit. E has the best results than Vit. C and yeast. The best concentration of Vit. C to achieve results is 0.06 mg/ml Ham’s F10.