The fatty acid composition of Streptomyces fulvoviolaceus 818 producer of antibiotic complex was analysed by capillary gas chromatography. The strain formed significant amounts of 18:2, 16:0 and 18:1 fatty acids. The culture synthesized very long chain fatty acids in the range from 20 to 27 carbon atoms. Among them more abundant levels of 2O :0, 23:0 and 24:0 acids were detected.Their occurance might be connected with lipase activity of strain. The total sum of unsaturated fatty acids in S. fulvoviolaceus 818 is the highest, the amount of saturated straight-chain fatty acid ranked second.