Acinetobacter baumannii is an important opportunistic pathogen that is rapidly evolving toward
multidrug resistance and is involved in various nosocomial infections that are often severe. Carbapenems
are considered one of the very few antibiotics left to treat infections caused by this organism. The aim of
this work is to study the antibiotic resistance pattern of Acinetobacter species isolated from different sites
of nosocomial infections. Patients and Methods: Antibiotic resistance pattern for 30 clinical isolates
of Acinetobacter was determined by the Kirby Bauer disk diffusion method. Extended spectrum betalactamase
(ESBLs) production was detected by double disc synergy test. Isolates detected to be imipenem
resistant were tested for metallo β -lactamase (MBL) production by E test. Results: Extended spectrum
beta lactamase production is high (19/30) 63.3% among Acinetobacter species. Ten (33.3%) isolates are
found to be resistant to imipenem and meropenem by the disk diffusion method and 3/30 (10%) of them
are found to be MBLs producers. Conclusion: Acinetobacter spp. are resistant to many classes of
antibiotics. Production of ESBLs, and MBLs are responsible for the multidrug resistance of these
pathogens.