Background Chemokine receptor type-5 (CCR5)-Δ32, a 32-base pair deletion of the C–C CCR5 gene, is associated with slowed human immunodeficiency virus disease progression in heterozygotes and protection against infection in homozygotes between carriers and noncarriers of each genetic variant. Aim The present study aimed to investigate the frequency of the CCR5-Δ32 mutation in Egyptian breast cancer (BC) patients. Materials and methods We determined the genotypic frequency of wild and mutant variants of CCR5 in 40 BC patients and 20 healthy individuals using restriction fragment length polymorphism and reverse hybridization. Results We found the absence of heterozygous and homozygous mutant gene variants in both BC patients and controls. Conclusion No significant difference in the frequency of CCR5 genotypes and alleles between patients and controls and no association between that gene and BC