Background: COVID-19 is an international health emergency of global concern. Physicians and nurses are more susceptible to COVID-19 infection. Therefore, doctors and nurses on the front lines must have essential COVID-19 awareness, knowledge, and preparedness. Objectives: To assess awareness, practices and preparedness level among healthcare workers at Benha university hospital (BUH) to fight against Covid-19 and to inspect environmental readiness. Methods: This was a cross-sectional study. Data were gathered using questionnaire which were filled out by physicians and nurses. The questionnaire included questions about respondents' demographics and 39 questions concerning knowledge, practices, preparedness and barriers. Overall, 175 physicians and 150 nurses had participated in the study. The observational environmental checklist included 49 questions. Results: About 80.6% of physicians and 71.3% of nurses show satisfactory score of knowledge about Covid-19. About 70.9% of physicians had good score of practices while near half of nurses (46%) had poor score of practices towards COVID-19. About 73.1% of physicians and 60% of nurses had adequate preparedness. There was a significant variation between score of practices and preparedness of physicians and nurses(p