The main study applied on the biochemical effects of probiotics on hepatic Lipid Metabolism experimentally induced Nonalcoholic fatty liver disease [NAFLD] in rats. Thirty male albino rats were divided into three groups [10 rats each]. The first group fed a normal diet and represents the control group. The second group [NAFLD] fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive control [+ ve control]. The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and probiotics [BIO-BC™] at a dose of 1-2g/liter at a rat dose[0.5-1ml/kg. body. weight]. Samples collected after 2,4and6 weeks after induction from treatment. Serum was collected for estimation of serum Tumor necrosis factor alpha [TNFα] , Interlukein-6 [IL-6] .Our results showed a significant increase in serum TNFα, IL-6. The behavioral biochemical results indicated treatment with probiotics showed significant changes and improved these parameters.