The main objective of this study to investigate the Beneficial effect of probiotics on Oxidative Stress in experimental induced Nonalcoholic fatty liver disease [ NAFLD] in rats . Thirty male albino rats were divided into three groups [10 rats each]. The first group fed a normal diet and represents the control group. The second group [NAFLD] fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive group [+ve control]. The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and probiotics [BIO-BC™]at a dose of 1- 2g/liter at a rat dose [0.5-1ml/kg. body. Weight] orally . Samples collected after 2,4and6 weeks after induction from treatment. serum was collected for estimation of Serum Nitric Oxide [ NO], Serum L- Malonialdehyde [L-MDA] and Tissues as liver [Enzymatic Antioxidants as [Liver Catalase [ L-CAT, Liver superoxide dismutase [L-SOD] ,Non Enzymatic Antioxidants as Liver Reduced Glutathione [L-GSH]. Our results revealed a significant decrease in serum NO, L-CAT, L-SOD, L-GSH moreover serum L-MDA. The behavioural biochemical results indicated treatment with probiotics showed significant changes and improves these parameters.